畜牧与饲料科学2012，Vol.33Issue(4)：4-6,3.
顶空固相微萃取—气相色谱法测定木糖发酵液中乙醇
Determination of Ethanol in Fermentation Solution of Xylose by Headspace Solid Phase Microextraction-gas Chromatography
摘要
Abstract
A headspace solid phase microextraction（HS-SPME） followed by gas chromatography had been developed for the determination of ethanol in fermentation solution of xylose.Various parameters including the type of fibers,extraction time,extraction temperature,pH value,ionic strength and desorption conditions were investigated for the optimization of HS-SPME performance for ethanol in fermentation solution of xylose.The high purity nitrogen with a flow rate of 4.0 mL/min,and DB-ALC2 capillary column（30 m×0.53 mm,2 μm） were used for separation.The oven temperature was programmed at 40 ℃.Under the optimal conditions,the quantitative results showed that the linear range of ethanol was 50-300 μg/L by using tert-butyl alcohol as internal standard.The detection limit（3σ） was 4.19 μg/L.The relative standard deviations for the determination of studied compound was less than 6%,and the recovery range was ranged from 91.01% to 97.90%.The proposed method had been successfully applied to determine ethanol in fermentation solution of xylose with satisfactory results.关键词
顶空固相微萃取/气相色谱/木糖发酵液/乙醇/内标法Key words
HS-SPME/GC/fermentation solution of xylose/ethanol/internal standard method分类
化学化工引用本文复制引用
贾鹏禹,孙蕊,张丽萍,李良玉,孙大庆,李洪飞,于伟..顶空固相微萃取—气相色谱法测定木糖发酵液中乙醇[J].畜牧与饲料科学,2012,33(4):4-6,3.基金项目
项目来源：黑龙江省科技厅“十一五”重点科技攻关项目（GC09A511）. （）
