农业环境科学学报2012，Vol.31Issue(4)：728-735,8.
不同栽培环境下有机与常规蔬菜土壤的细菌群落多样性差异
Bacterial Community Diversity of Vegetable Soils in Organic and Conventional Managements Under Different Cultivated Environments
摘要
Abstract
Bacterial community plays a significant role in vegetable cultivation. In this study, PCR-DGGE method was used to study patterns of bacterial community diversity under organic versus conventional management in horticultural soil based on 16S rDNA/rRNA. Also its relationship with soil physicochemical properties was researched. The results indicated that soil bacterial diversity has a significant change under different managements and sample period as analysis DGGE gels. Moreover, the abundance of each dominant band varied among each treatment during sample period. On the other hand, by observing 16S rRNA DGGE fingerprint we found that there is less variability than 16S rD-NA fingerprint has, and 16S rRNA DGGE fingerprint can be grouped separately in terms of organic and conventional management by UPG-MA. Additionally, analysis of 16S rDNA DGGE fingerprint indicated a seasonal influence on bacterial community. From 26 different bands which were excised from DGGE gel, the result showed us that there were 9 bands belong to Pseudomonas and the rest of bands belong to uncultured bacterium clones. Shannon-Wiener index showed organic management promoted bacterial diversity of soil more intensively than conventional management does. According to the figure drew by CCA, it points out soil pH is the strongest environmental factor which can influence soil bacterial diversity. Meantime, the level of microbial mass C, N and organic matter had a significant increase under organic management. On the other side, it indicated that soil of greenhouse had a higher humidity than soil from open field. During the same period, pH value was higher in organic management soil than it under conventional management virtually. In sum, organic management can diversify active bacterial community and have beneficial ecological effects.关键词
蔬菜土壤/有机栽培/常规栽培/温室/露地/PCR-DGGE/细菌多样性Key words
vegetable soils/ organic management/ conventional management/ greenhouse/ open field/ PCR-DGGE/ bacterial diversity分类
农业科技引用本文复制引用
叶俊,Pablo Gonzalez Perez,王小丽,黄丹枫..不同栽培环境下有机与常规蔬菜土壤的细菌群落多样性差异[J].农业环境科学学报,2012,31(4):728-735,8.基金项目
上海市科委设施蔬菜清洁高效生产与环境质量保障技术研究与示范项目(09391910400) （09391910400）
上海市农委设施园艺高效生态生产关键技术研究与示范项目(沪农科攻字20080803,沪农推字20090102) （沪农科攻字20080803,沪农推字20090102）
农业部行业项目(200903056) （200903056）
上海市蔬菜学重点学科项目(B209) （B209）
