农业环境科学学报2012，Vol.31Issue(4)：843-847,5.
添加木质素降解菌对堆肥中酶活性的影响
Effect of Inoculating Lignin Degradation Strains on Enzymic Activities in Composting
摘要
Abstract
Composting is the process under which substrate is decomposed and converted into stable and environment-friendly organic matter, therefore it is a common technology for disposing of agricultural waste at present. Agricultural waste contains large quantities of lignocel-lulose, which mainly consists of lignin that is difficult to be biodegraded due to its complex component and structure and leads to the insufficient transformation of humus and low quality of compost. In this sense,how to accelerate the decomposition and humification of lignin is critical to the sufficient maturity and the quality of compost. Cow dung and straw were compost under static aerobic condition and lignin degrading strains were inoculated in the pile. Changes in temperature, pH and lignin-degrading enzymes activity during composting were investigated in order to assess the influence of the inoculation on composting from the perspective of biological enzyme. The result indicated that: the inoculated compost was characterized by reaching high temperature phase 2 days earlier and keeping this period for 12 days longer than the control.In the first 8 days, pH value rose more rapidly and was higher and faster than control and C/N ratio reached 20:1 5 days earlier than control, indicating maturity of the compost. The results of enzymatic activities analyse in composting showed that after adding inoculant, (3- glucosidase enzymes reached the first peak activity 14.7 μmol on the sixth day, 6 days earlier than the control and also 12.3 μmol higher than it was at the peak; Sodium carboxymethyl cellulose enzyme reached its peak (3 270 U) on the twelfth day, 1 220 U higher than control; The peak activity for laccase enzyme was 93.5 U, and the peak of CK was only 82.8 U; And the manganese peroxidas enzyme activity was 75.25 U in the high temperature period, however, the highest activity of the control was only 54.8 U.Thus it could be deduced thaf inoculating exogenous microbes could raise the enzyme activity involved, increase the temperature,accelerate the decomposition, improve the utilization of microbial substrate, and thereby enhance the composting efficiency.关键词
堆肥/木质素/微生物菌剂/酶活性Key words
compost/ lignin/ microbial inoculant/ enzymatic activities分类
农业科技引用本文复制引用
张晓倩,许修宏,王晶,刘佳..添加木质素降解菌对堆肥中酶活性的影响[J].农业环境科学学报,2012,31(4):843-847,5.基金项目
科技部十一五科技支撑项目(2008BAD95B06-02-04) （2008BAD95B06-02-04）
科技部十一五科技支撑项目(2008BADA1B01) （2008BADA1B01）
林业公益性行业科研专项(201104048) （201104048）
哈尔滨市科技攻关计划项目(2007AA6CN105) （2007AA6CN105）
