农业生物技术学报2012，Vol.20Issue(6)：606-615,10.DOI:10.3969/j.issn.1674-7968.2012.06.003
优质新疆拉面品种品质评价的多重PCR体系构建与应用
Establishment and Application of Multiplex PCR Systems for Cultivar Evaluation of High Quality Xinjiang Hand Stretched Noodle
摘要
Abstract
Xinjiang hand stretched noodle (XHSN) is one of the most popular noodles in China, and evaluation for high quality XHSN is essential to success of breeding program. Based on past research achievement of the XHSN and relevant molecular markers for the genes coding high quality XHSN, three types of multiplex PCR systems were developed and validated with 13 Xinjiang winter wheat cultivars and advanced lines with known genes, and used to evaluate the genes associated with XHSN quality in 46 Xinjiang winter wheat {Triticum aestivum L.) cultivars and advanced lines. The first multiplex PCR was used to simultaneously detect genes Ax2*, Pinb-Dlb and w-secalin, the second one was to detect the genes Psy-Ala, Psy-A lb, Psy-A lc, Ppo-Dla and Ppo-Dlb, and the third one was to detect Glu-B3a, Wx-Bla and Wx-Blb. The test results showed that the frequencies of genes Ax2*, Pinb-Dlb, without w-secalin, Psy-Ala, Psy-Alb, Psy-A lc, Ppo-Dla, Ppo-Dlb, Glu-B3a, Wx-Bla and Wx-Blb in the 46 winter wheat cultivars and advanced lines were 30.4%, 45.7%, 78.3%, 94.5%, 4.3%, 2.2%, 91.3%, 8.7%, 21.7%, 91.3% and 8.7%, respectively. Five (Ppo-Dla, without w-secalin, Pinb-Dlb, Glu-B3a and Ax2*) of those genes related with the high quality for the XHSN in Xinjiang wheats had higher frequencies than those in wheats from other Chinese regions, but the high quality genes Psy-A lb and Wx-Blb had lower frequencies. Meanwhile, 19 combinations of the high quality genes appeared in the tested cultivars and advanced lines, the combinations with one, two, three, four or five high quality genes accounted for 13.0%, 32.6%, 26.1%, 23.9% and 4.3%, respectively. The cultivar with six or seven high quality genes was not found. In all combinations of the high quality genes, Ppo-D1a/without w-secalin, Ppo-DlaJGlu-B3aIwithout w-secalin and Ppo-Dla/Ax2*/Pinb-Dlb/without w-secalin had the highest frequencies. Those results showed that the three systems developed in this study were effective and stable to amplify target bands for these genes and could be used to evaluate the XHSN quality quickly. In order to improve the quality of the XHSN in the future, those high quality genes and their combinations should be maintained for the XHSN. Developing cultivars with lower in both yellow pigment (Psy-A lb) and amylose (Wx-Blb) contents will be important objectives in the XHSN wheat breeding programs in the future. Developed three multiplex PCR systems and identified wheat genetic resources with the high quality genes in this study can be provide valuable information for estimation and breeding of the XHSN cultivars in the future.关键词
新疆拉面/品质评价/分子标记/多重PCR/新疆冬小麦Key words
Xinjiang hand stretched noodle/ Quality evaluation/ Molecular marker/ Multiplex PCR/ Xinjiang winter wheat引用本文复制引用
王晓龙,桑伟,谢敏娇,相吉山,张晓科,穆培源,高正,王宏礼..优质新疆拉面品种品质评价的多重PCR体系构建与应用[J].农业生物技术学报,2012,20(6):606-615,10.基金项目
兵团科技支疆计划(No.2008ZJ10)、国家自然科学基金项目(No.30560078和U1178306)、兵团农业科技攻关计划(No.2011BA002)、农业部“948”研究项目(No.2006-G2)、小麦产业技术体系建设专项(No.nycytx-03)和西北农林科技大学唐仲英育种基金 （No.2008ZJ10）
