农业生物技术学报2012，Vol.20Issue(6)：627-635,9.DOI:10.3969/j.issn.1674-7968.2012.06.005
甘蓝胞质雄性不育(OguCMS)相关的MYB转录因子BoMYB1的克隆与表达分析
Cloning and Expression of an Ogura Cytoplasmic Male Sterile (OguCMS)-related MYB Transcription Factor in Brassica oleracea var.capitata
摘要
Abstract
Ogura cytoplasmic male sterile (OguCMS) is the most widely used male sterile type in cabbage breeding. MYB transcription factors play a key role in regulation of plant defense response and multiple development processes. In present experiment, a R2R3-MYB transcription factor which down regulated 10.3 times in cabbage (Brasska okracea var. capitata) OguCMS lines was cloned by SMART RACE strategy.The full-length cDNA of B0MYB1 was 1 141 bp, which contained a 196 bp long 5' untranslated region, a 246 bp long 3' untranslated region and a 699 bp long open reading frame (GenBank accession number: JN703995). It was localized in the nucleus by subcellular localization prediction. It was an anther preferentially expressed gene in cabbage, which reached its expression peak in the late development. It was induced by the regulation of plant hormones salicylic acid(SA) and jasmonate methyl (JA-ME), and consequently regulated the expression of anther development genes. The experimental results suggests that B0MYB1 may be one of the important genes which involved in OguCMS anther development.关键词
甘蓝/MYB转录因子/RACE技术/序列分析/激素应答Key words
Cabbage/ MYB transcription factor/ RACE/ Sequence analysis/ Hormone response引用本文复制引用
张磊,康宗利,刘海霞,康俊根..甘蓝胞质雄性不育(OguCMS)相关的MYB转录因子BoMYB1的克隆与表达分析[J].农业生物技术学报,2012,20(6):627-635,9.基金项目
“十二五”国家科技支撑计划项目(No.2012BAD02B01)、国家自然科学基金(No.31171958)和北京市自然科学基金项目(No.6102012) （No.2012BAD02B01）
