热带作物学报2012，Vol.33Issue(3)：446-452,7.DOI:10.3969/j.issn.1000-2561.2012.03.010
大豆GmPic基因的分离及表达分析
Cloning of the Photoperiod Response Gene GmPic from Soybean and Expression Analysis
摘要
Abstract
In this study, the light-sensitive soybean variety "ZhongDou 24" was processed for a long day, short day photoperiod and natural light, and then the differential expression fragments were screened via cDNA桝FLP. The full length of differential gene GmPic was obtained by RACE technology. The gene GmPic is 983 bp, which contains a complete open reading frame encoding 248 amino acids. The gene in the genetic relationship is highly homology to the mitochondrial phosphate transport protein amino acids. Finally, expression profile of the gene GmPic in leaves treated with different photoperiods was carried out by semi-quantitative RT-PCR. The expression pattern analysis showed that the expression of the gene GmPic is inhibited by short-day in an early stage of soybean's growth and development; while it is enhanced by long-day. So the gene GmPic of soybean mitochondrial phosphate transporter gene may serve as a negative regulator of photoperiodic response in soybean. This study of soybean mitochondrial phosphate transporter gene was conducted to further clarify the molecular mechanism of soybean photoperiod response.关键词
大豆/线粒体磷酸盐转运蛋白/光周期/cDNA-AFLPKey words
Soybean(Glycine max)/Mitochondrial phosphate transport protein/Photoperiod/cDNA-AFLP分类
农业科技引用本文复制引用
吴辉,符秀梅,张巧玲,郭倩丽,陈银华,沙爱华..大豆GmPic基因的分离及表达分析[J].热带作物学报,2012,33(3):446-452,7.基金项目
高等学校博士点专项基金(新教师类)(No.20094601120001) （新教师类）
海南省研究生创新科研课题基金(No.Hys2010-1). （No.Hys2010-1）
