热带作物学报2012，Vol.33Issue(3)：453-458,6.DOI:10.3969/j.issn.1000-2561.2012.03.011
农杆菌介导的水稻Waxy基因过量表达初步研究
Over-expression of Waxy Gene on Rice Mediated by Agrobacterium
摘要
Abstract
To obtain high amylose transgenic rice, the functions of Waxy genes in amylose synthesis in rice were studied. The Waxy gene was cloned by the technique of RT-PCR, the vector of over-expression of Waxy genes was constructed. The target gene was transformed into rice using rice genetic transformation system mediated by Agrobacterium. It showed that exogenous genes had been successfully transformed into rice by the mean of the single-copy insertion by PCR detection and real-time quantitative PCR. The results showed the exogenous Waxy gene had been successfully expressed, and the expression amount was improved obviously than the control. It would become the foundation of the further study of the gene function and regulation of amylose synthesis in rice that the vector of over-expression of Waxy gene was transformed successfully.关键词
水稻/Waxy基因/过量表达/遗传转化Key words
Rice/ Waxy gene/Over-expression/Transformation分类
生物科学引用本文复制引用
汪结明,张建,程备久..农杆菌介导的水稻Waxy基因过量表达初步研究[J].热带作物学报,2012,33(3):453-458,6.基金项目
国家高技术研究发展计划(863计划)项目(No.2008AA10Z408). （863计划）
