热带作物学报2012，Vol.33Issue(3)：475-478,4.DOI:10.3969/j.issn.1000-2561.2012.03.015
不同基本培养基和外植体对剑麻愈伤组织诱导及分化的影响
Effects of Different Explants and Basic Mediun on Callus Induction and Differentiation of Agave sisalana
摘要
Abstract
The effects of four kinds of basic media and three kinds of sisal explants on the callus induction and differentiation of A gave sisalana were studied. The results showed that 5H had the best effect on induction and differentiation of callus. The ratio of callus was almost 100%, the weight of callus was very large and the ratio of differentiation is 21.1%. Sisal leaves had the best effect on induction of callus with the best ratio and the largest weight of callus. Sisal had low demand for nitrogen throughout the process of callus induction and differentiation, and lower concentration of ammonium salt was advantageous to induction and differentiation of callus. Higher concentration of hydrochloric acid thiamine (vitamin B1) in the media increased callus induction and differentiation significantly. It seemed that Cu, Mo, Co and inositol were not essential to the induction of sisal callus, and they may be very favorable for callus differentiation.关键词
基本培养基/外植体/剑麻/愈伤组织/诱导/分化Key words
Basic medium/Explanls/Agave sisalana/Callus/Induction/Differentiation分类
生物科学引用本文复制引用
杨峰,刘巧莲,代真真,陈河龙,高建明,郑金龙,易克贤..不同基本培养基和外植体对剑麻愈伤组织诱导及分化的影响[J].热带作物学报,2012,33(3):475-478,4.基金项目
中央级公益性科研院所基本科研业务费专项(No.1TBB110213) （No.1TBB110213）
“948”项目(No.2010-Z6) （No.2010-Z6）
国家麻类产业技术体系建设专项资金(No.CARS-19). （No.CARS-19）
