日用化学工业2012，Vol.42Issue(2)：115-119,5.
桦褐孔菌总黄酮的微波提取及美容功效研究
Study of microwave-assisted extraction and cosmetic effect of total flavonoids from Inonotus obliquus (Fr.) Pilat
摘要
Abstract
Extraction technology of total flovonoids from Inonotus obliquus ( Fr. ) Pilat were optimized via orthogonal designed experiment, and tyrosinase inhibition rate of the extract was measured. Varnishing cream was prepared using the extract as the active additive. Various performance tests of the varnishing cream were conducted, including determination of malonaldehyde (MDA) content, determination of SOD activity in mouse skin via pyrogallol auto - oxidation test and determination of hydroxy free radical scavenging capacity of skin tissue in vitro. Experimental results showed that the optimum conditions for extraction of flavinoids from Inonotus obliquus (Fr. ) Pilat are as follows: ethanol as solvent with volume fraction of 70% ; ratio of Inonotus obliquus (Fr. ) Pilat material to. solvent (g: mL) , 1: 40;microwave treatment time, 120 s;microwave power, 352 W. Under such conditions, the extraction yield of flavonoids from Inonotus obliquus (Fr. ) Pilat achieves 4. 96%. When vanishing cream base was treated by addition of 7. 5 mL of the extract with mass concentration of 0. 024 g · L-1 Inonotus obliquus ( Fr. ) Pilat flavonoids, the tyrosinase inhibition rate achieves the best condition of 25.0% and the finished product shows good appearance and stability. In addition,the antioxidation test results showed that the extract could reduce the formation of MDA, increase the enzymatic activity of SOD and hydroxyl free radical scavenging capacity in mouse skin tissue and displays good dosage -effect relationship. All results indicate that Inonotus obliquus ( Fr. ) Pilat flavonoids has certain antioxidation capacity and tyrosinase inhibition effect;it can be used as a cosmetic additive for skin decrepitude retarding and whitening.关键词
桦褐孔菌总黄酮/提取/抗衰老/美白Key words
flavonoids from Inonotus obliquus (Fr. ) Pilat/extraction/decrepitude retarding/whitening分类
化学化工引用本文复制引用
蔡建秀,龚丽芬,许丽璇,陈伟伟..桦褐孔菌总黄酮的微波提取及美容功效研究[J].日用化学工业,2012,42(2):115-119,5.基金项目
福建省科技厅重点资助项目(2009N0049) （2009N0049）
福建省教育厅科技资助项目(JA11352) （JA11352）
泉州师范学院福建省高校服务海西建设重点资助项目(A101) （A101）
泉州师院开放实验室资助项目(20110111) （20110111）
泉州师院大学生创新实验资助项目(2011CXSY校02) （2011CXSY校02）
