日用化学工业2012，Vol.42Issue(3)：196-200,5.
α-淀粉酶在MCM-41介孔分子筛上的固定化研究
Immobilization of α - amylase on MCM -41 mesoporous molecular sieves
摘要
Abstract
A kind of α - amylase was immobilized on MCM -41 mesoporous molecular sieves with dipping method. Influence of factors such as adsorption time,initial dosage of enzyme and pH during the immobilization process on the α - amylase immobilization performance as well as the immobilized α - amylase activity, stability,carrier structure were studied. Results showed that when the adsorption time is 11 h,the initial dosage of enzyme is 70 mg · g-1 ,and the pH is kept at 5. 9,recovery of activity of the immobilized enzyme achieves 48%. As compared with the free (non - immobilized) enzyme,the heat resisting capacity of the immobilized enzyme is enhanced. At 70 ℃, relative activity of the immobilized enzyme achieves 75% , while that of free enzyme achieves 14% only. When pH ranges between 3.3 ～ 8. 0, relative activity of the immobilized enzyme achieves 62% - 100% ,while the free enzyme achieves 5% ～ 100%. Obviously,the immobilized enzyme offers broader adaptability for pH. In addition,the storage stability of immobilized α -amylase is obviously improved. The immobilized α - amylase can be used repeatedly for certain times and the carrier after the immobilization still maintains good mesoporous structure.关键词
α-淀粉酶/介孔分子筛/MCM-41/固定化Key words
α - amylase/ mesoporous molecular sieve/ MCM - 41/ immobilization分类
生物科学引用本文复制引用
王亮亮,刘雪松,宋伟明,邓启刚..α-淀粉酶在MCM-41介孔分子筛上的固定化研究[J].日用化学工业,2012,42(3):196-200,5.基金项目
黑龙江省自然科学基金资助项目(B201010) （B201010）
