日用化学工业2012，Vol.42Issue(3)：201-204,4.
抑制非酶糖基化反应体外实验方法的建立及应用
Establishment and application of in vitro experimental method for inhibition of non - enzyme glycosylation
摘要
Abstract
Reaction conditions for in vitro non - enzyme glycosylation (NEG) were studied via single factor analysis. Optimal conditions were found as follows: 1.7 g bovine serum albumin and 1.0 g glyoxal is to be added to 100 mL phosphate buffer in turn;and 0. 2 g sodium azide is to be added as preservative. The mixture is incubated at 37 ? for 72 h. Optimal conditions for fluorescence detection were as follows; excitation wavelength is 440 nm with 10 nm slit,emission wavelength is 480 nm with 10 nm slit,and detection range is from 460 to 600 nm. Twelve plant extracts were detected by NEG method in vitro. Tea polyphenols, Portulaca oleracea extract and Magnolia officinalis extract show stronger inhibiting efficacy (99. 5% , 99. 0% and 97. 6% ). Portulaca oleracea extract and Magnolia officinalis extract were selected for evaluation by LAB method in vivo and the results are consistent with that of NEG method in vitro.关键词
去黄亮肤/非酶精基化/体外法/马齿苋提取物/厚朴提取物Key words
skin lightening/non -enzyme glycosylation (NEG) /in vitro/Portulaca oleracea extract/Magnolia officinalis extract分类
化学化工引用本文复制引用
宋丽雅,来吉祥,信璨,魏少敏,董银卯,何聪芬..抑制非酶糖基化反应体外实验方法的建立及应用[J].日用化学工业,2012,42(3):201-204,4.基金项目
北京工商大学学科与研究生教育-化妆品科学与技术学科建设资助项目(PXM2012-014213-000068) （PXM2012-014213-000068）
