四川理工学院学报：自然科学版2012，Vol.25Issue(3)：5-8,4.
PCR—DGGE用于浓香型大曲微生物群落分析的条件优化
Optimization of Denaturing Gradient Gel Electrophoresis for Microbial Communities in Luzhou-flavor Daqu
摘要
Abstract
Ploymerase chain reaction and denaturing gradient gel electrophoresis （PCR-DGGE） technique is used to reveal the Microbial Communities in Luzhou-flavor Daqu. In order to improve the accuracy, sensitivity and repeatability of DGGE, the running parameters of DGGE are optimized by level electrophoresis and electrophoresis time. The results show that the best range of denaturing gradients is 35% ~ 55% of DGGE of bacteria; the best range of denaturing gradients is 30% ~ 50% of DGGE of fungi ; the best eleetrophoretic time is 16 hours through the time process method. The Daqu microbial com- munity of different periods is researched by the optimized PCR-DGGE technology,and rich microbial diversity is obtained.关键词
浓香型大曲/微生物/PCR—DGGE/变性剂Key words
Luzhou-flavor Daqu/microorganism/PCR-DGGE/denaturant分类
生物科学引用本文复制引用
王世宽,侯华,袁城金,谢仁有,洪玉程..PCR—DGGE用于浓香型大曲微生物群落分析的条件优化[J].四川理工学院学报：自然科学版,2012,25(3):5-8,4.基金项目
基金项目：四川省科技厅自然科学基金项目 （）
