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泸型大曲中根霉固态发酵产糖化酶条件研究

黄丹 尚志超 袁先铃 李玉龙

四川理工学院学报:自然科学版2012,Vol.25Issue(3):9-13,5.
四川理工学院学报:自然科学版2012,Vol.25Issue(3):9-13,5.

泸型大曲中根霉固态发酵产糖化酶条件研究

Study on the Conditions of Glucoamylase Production of the Rhizopus Oryzae in Luzhou-flavor Daqu by Solid State Fermentation

黄丹 1尚志超 1袁先铃 1李玉龙1

作者信息

  • 1. 四川理工学院生物工程学院,四川自贡643000
  • 折叠

摘要

Abstract

The conditions of glucoamylase production of the Rhizopus oryzae in Luzhou - flavor Daqu by solid state fer- mentation are studied. By single factor experiment and saccharifying enzymic activityas as an assessing index, the influence on saccharifying enzymic aetivityas of the water content in culture medium, the culture time, the culture temperature are stud- ied. According to the Box-Benhnken design, the conditions of glucoamylase production of the Rhizopus oryzae are optimized by solid state fermentation. The results show that the optimum conditions of glucoamylase production of the Rhizopus oryzae in Luzhou-flavor Daqu by solid state fermentation are the culture temperature 29℃, the water content in culture medium 53%, culture time 164h, glucoamylase activity was 2 194.44U/g.

关键词

根霉/糖化酶/固态发酵/产酶条件多相流

Key words

Rhizopus oryzae/glucoamylase/solid state fermentation/conditiones of glucoamylase production

分类

生物科学

引用本文复制引用

黄丹,尚志超,袁先铃,李玉龙..泸型大曲中根霉固态发酵产糖化酶条件研究[J].四川理工学院学报:自然科学版,2012,25(3):9-13,5.

基金项目

基金项目:川菜发展研究中心项目 ()

泸州老窖科研基金项目 ()

酿酒生物技术及应用四川省重点实验室项目 ()

四川理工学院研究生创新基金项目 ()

四川理工学院学报:自然科学版

2096-7543

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