四川师范大学学报（自然科学版）2012，Vol.35Issue(3)：386-390,5.DOI:10.3969/j.issn.1001-8395.2012.03.022
氯化血红素与牛血清白蛋白的作用及表面活性剂对其的影响研究
Investigation on the Interaction of Hemin with Bovine Serum Albumin and the Effect of Surfactants on the Reaction
摘要
Abstract
The interaction of hemin with bovine serum albumin (BSA) has been investigated by fluorescence method in the aqueous solution and the surfactant solutions in the paper. According to experiment data, the binding constants, binding sites and thermodynamics constants were obtained at different pH, temperature and surfactant solutions by Stem-Volmer equation, Lmeweaver-Burk equation and thermodynamics equation. The parameters of Ka, n, △G, △H and T△S are 1. 35 × 107, 1. 32, -41. 36 kJ· mol-1, -51.45 kj· mol-1 and - 10.09 kJ · mol-1, respectively, in the aqueous solution at pH 7.00 and 303 K. The results show that the fluorescence of BSA is quenched regularly by the hemin, and the mechanism of the fluorescence quench is investigated. The hydropho-bic interaction force plays a main role in the binding of hemin with BSA. The wavelength of the fluorescence spectrum shifts to shortwave and its intensity decreases in the surfactant solutions at 346 nm.关键词
氯化血红素/牛血清白蛋白/表面活性剂/荧光光谱Key words
hemin/ bovine serum albumin/ surfactant/ fluorescence quench分类
化学化工引用本文复制引用
刘凡,张元勤..氯化血红素与牛血清白蛋白的作用及表面活性剂对其的影响研究[J].四川师范大学学报（自然科学版）,2012,35(3):386-390,5.基金项目
四川省教育厅自然科学基金(08ZC038)资助项目 （08ZC038）
