水产学报2012，Vol.36Issue(4)：503-508,6.
利用cDNA-AFLP技术研究副溶血弧菌感染下拟穴青蟹的基因差异表达
The differential gene expression of Scylla paramamosain upon Vibrio parahaemolyticus infection by cDNA-AFLP
李升康 1朱烁 1张昭 1温小波 1李远友1
作者信息
摘要
Abstract
Mud crab Scylla paramamosains is widely cultured in brackish and seawater ponds along the coast of southeast China including Shantou. In recent years, mud crab cultured in Niutianyang reclaiming coastal area of Shantou suffered from serious diseases, especially the bacterial diseases (such as Vibrio parahaemolyticus), which results in high mortality and great economic loss. In present study, V.parahaemolyticus isolated from Shantou Niutianyang crab culture area was injected to 5.paramamosains to study the differential gene expression of the mud crab by cDNA-AFLP technology. Furthermore, 20 fragments that exhibited differential expression were discovered and sequenced. The results show that 6 fragments are homologous to the known functional genes, 5 of which are up-regulated. They are involved in energy metabolism including arginine kinase, phosphoenolpymvate-carboxykinase and glyceraldehydes 3 phosphate dehydrogenase, arginyl-tRNA synthetase involved in transport progress, as well as the chymotrypsin-like proteinase involved in defense response. The other 8 fragments were identified to be unknown function genes. The result here shows that the response of S.paramamosain upon V. parahaemolyticus infection is involved in multiple pathways and needs further investigation.关键词
Scylla paramamosain/ Vibrio parahaemolyticus/ differential gene expression/ cDNA-AFLPKey words
Scylla paramamosain/ Vibrio parahaemolyticus/ differential gene expression/ cDNA-AFLP引用本文复制引用
李升康,朱烁,张昭,温小波,李远友..利用cDNA-AFLP技术研究副溶血弧菌感染下拟穴青蟹的基因差异表达[J].水产学报,2012,36(4):503-508,6.
