水产学报2012，Vol.36Issue(4)：522-528,7.DOI:10.3724/SP.J.1231.2012.27749
重金属离子Cd2+对泥蚶鳃及肝脏细胞显微和超微结构的影响
Effects of cadmium on the microstructure and ultrastructure of gill and hepatopancreas in Tegillarca granosa
摘要
Abstract
The aim of this paper is to investigate the toxic influence of cadmium on the histopathological alterations of gill and hepatic cells in Tegillarca granosa exposed to various concentrations of cadmium(5 μg/L, 15 μg/L, 45 μg/L, 90 μg/L). The experiment lasted for 96 hours. The results are as follows: as the metal concentration rose, the gill cavity swelled, in which stored lots of haemocytes; finally the gill broke and dissolved, where the epithelia cells dropped; the findings of ultrastructure showed that there was granular osmiophilic material in gill epithelia cells, moreover, the number of secondary lysosome and mitochondrion increased, and the cells became vacuolated. The influnce of cadmium on the microstructure of hepatic cells was not obvious, only turning up some yellow sediment. However, its ultrastructure results indicated that the granular osmiophilic material was also present, and the secondary lysosome increased as well, consistent with the cases in gill; Furthermore, the nucleus of hepatic cells deformed and shrank, even showed vacuoles, suggesting the cells were irreversibly damaged. But the two most sensitive organelle, mitochondrion and endoplasmic reticulum, were normal, so we infer that the results may be caused by antioxidant enzymes, which were induced by cadmium.关键词
泥蚶/镉/鳃/肝/超微结构Key words
Tegillarca granosa/ cadmium/ gill/ hepatopancreas/ ultrastructure分类
资源环境引用本文复制引用
陈彩芳,沈伟良,霍礼辉,刘浩明,林志华..重金属离子Cd2+对泥蚶鳃及肝脏细胞显微和超微结构的影响[J].水产学报,2012,36(4):522-528,7.基金项目
国家科技支撑计划(2009BADB7B02) （2009BADB7B02）
国家贝类产业技术体系(CARS-48) （CARS-48）
宁波市自然科学基金(2011A610016) （2011A610016）
浙万院省重中之重学科开放基金(KF2011005) （KF2011005）
浙江省大学生科研创新团队项目 （）
