水产学报2012，Vol.36Issue(4)：538-545,8.DOI:10.3724/SP.J.1231.2012.27713
氨氮胁迫对青鱼幼鱼鳃丝Na+/K+-ATP酶、组织结构及血清部分生理生化指标的影响
Effects of ammonia stress on the gill Na+/K+-ATPase, microstructure and some serum physiological-biochemical indices of juvenile black carp(Mylopharyngodon piceus)
摘要
Abstract
An important environmental concern associated with intensive fish culture is the production of ammonia, which is formed through ammonification of organic matter like unconsumed food and feces. The object of this study was to evaluate the effects of ammonia-N stress on the gill Na+/K+-ATPase, micro-structure and serum physiological-biochemical indices of juvenile black carp, Mylopharyngodon piceus. The black carp, whose initial average weight was (7.00 ±0.14) g, were transferred off the freshwater and exposed to different ammonia-N levels: 0 mg/L (low, control), 10 mg/L (middle) and 20 mg/L (high) NH4CI, each ammonia-N level was randomly triplicate and sampled at 0, 6, 12, 24, 48 and 96 h, respectively. The results indicated that the gill Na+/K+-ATPase activities in the middle and high ammonia-N treatment groups decreased gradually at first and reached the minimum at 12 h, 6 h, respectively, then in-creased gradually and reached the maximum at 48 h, and returned to the same level as that of control group after 96 h. Gill microstructure observation showed that chloride cell in middle ammonia group increased at 12 h; pavement cells fell off partly at 24 h; chloride cells vacuolated partly, blood capillary fractured and red blood cell overflowed at 96 h. But in high ammonia group, chloride cell increased at 6 h; pavement cells fell off partly at 12 h and largely at 24 h; After 96 h, blood capillary fractured on the base region of the lamellae and congestion, pillar cell disorganized of arrangement. Serum cortisol and glucose concentration in ammonia-N treatment group increased gradually at first 12 h, and then decreased gradually and returned to the same level as that of control group after 48 h. The activities of total superoxide dismutase (SOD) in ammonia-N treatment group increased significantly at first 6 h (P<0.05), and SOD activity in the middle ammonia-N treatment group returned to the same level as that of control group after 12 h, but SOD activity in the high ammonia-N treatment group was still significantly higher than that of control group after 96 h (P<0.05). Serum catalase activity decreased at first 6 h and then increased and reached the maximum at 12 h, and then decreased and recovered to the same level as that of control group after 48 h. Total antioxidant capacity (T-AOC), glutathione (GSH) and alanine transaminase (ALT) activities in ammonia-N treatment group decreased gradually at first 24 h, and malondialdehyde (MDA) concentration showed converse trends. After 96 h, GSH and ALT activities in ammonia-N treatment group returned to the same level as that of control group, however, the MDA concentration in ammonia group was significantly higher than that of control group and T-AOC activity in high ammonia group was significantly lower than that of control group. These results indicated that the ammonia stress could seriously disturb the antioxidant system at short-term ammonia-N stress, but fish showed the self-regulation ability , however, the antioxidant capacity decreased and gill structure was damaged after long-term ammonia-N stress.关键词
青鱼/氨氮胁迫/Na+/K+-ATP酶/鳃组织/应激/抗氧化系统Key words
Mylopharyngodon piceus/ ammonia stress/ gill Na+/K+-ATPase/ gill structure/ stress/ antioxidant capacity分类
农业科技引用本文复制引用
胡毅,黄云,钟蕾,肖调义,文华,郇志利,毛小伟,李金龙..氨氮胁迫对青鱼幼鱼鳃丝Na+/K+-ATP酶、组织结构及血清部分生理生化指标的影响[J].水产学报,2012,36(4):538-545,8.基金项目
湖南省自然科学基金(11JJ3037) （11JJ3037）
国家大宗淡水鱼类产业技术体系(CARS-46-462) （CARS-46-462）
中国水产科学研究院淡水生态与健康养殖重点实验室开放课题(2010FEA03018) （2010FEA03018）
湖南省研究生创新课题(CX2010B285) （CX2010B285）
