水产学报2012，Vol.36Issue(5)：641-646,6.DOI:10.3724/SP.J.1231.2012.27788
牙鲆骨骼生长性状与微卫星标记的相关性分析
Correlation analysis of microsatellite DNA markers with skeletal growth traits of Japanese flounder (Paralichthys olivaceus)
摘要
Abstract
In this article, 106 microsatellite markers were used to analyze the genotype of sixty-two mitotic gynogenetic doubled haploids of Japanese flounder, Paralichthys olivaceus. Measurement results of body length and height were got by directly measuring skeleton during the process of X-ray imaging. Correlation and regression analysis of the body weight, length and height were conducted by utilizing SPSS 13.0 software. Least square method was used to analyze the effects of these 106 microsatellites on body weight, length and height of these 62 doubled haploids. Results revealed that 17 microsatellites were discovered to be very significantly related to body weight, length and height. During these 17 markers, Poli2042TUF, Polil980TUF, Poli2045TUF, Poli2039TUF, Poli34TUF, PoGT17, HLJYP45, HLJYP81 had a significant impact on body length (P<0.05), Polil02TUF, Polil36TUF, Poli62MHFS, 6-G3 had a significant impact on body height (P<0.05), Poli72HFSM , Polil06TUF had a significant impact on body weight and height (P<0.05), Poli2023TUF, Polil013TUF had a significant impact on body length and height (P<0.05), Po-li162TUF had a significant impact on body weight, length and height (P<0.05). These microsatellite markers had an advantageous effect on growth traits and these loci could be used as the reference markers for marker-assisted breeding of Japanese flounder.关键词
牙鲆/双单倍体/生长性状/骨骼/微卫星Key words
Paralichthys olivaceus/ doubled haploid/ growth trait/ skeleton/ microsatellite marker分类
生物科学引用本文复制引用
安泉泉,刘海金,王桂兴,刘永新,刘奕,司飞..牙鲆骨骼生长性状与微卫星标记的相关性分析[J].水产学报,2012,36(5):641-646,6.基金项目
国家鲆鲽类产业技术体系资助(CARS-50-G2) （CARS-50-G2）
国家公益性农业行业专项资助(200903046) （200903046）
