水产学报2012，Vol.36Issue(5)：647-651,5.DOI:10.3724/SP.J.1231.2012.27734
布氏石斑鱼的染色体核型、银染和C-带
A study on the karyotype, Ag-NORs and C-banding in Epinephelus bleekeri
摘要
Abstract
Epinephelus bleekeri is important in commercial fisheries of tropical and subtropical seas. It has been broadly cultured in many Asian countries, such as China, Japan, Vietnam and Malaysia.Despite many studies on its culture techniques, the detailed cytogenetic data of this species is still lacking. To add E. bleekeri cytogenetic data and lay the foundation for grouper chromosome evolution and breeding studies, the karyotype of E. bleekeri was studied in this paper using multiple methods, including Giemsa staining, Ag-NORs and C-banding. The following results were obtained: E. bleekeri had a diploid chromosome number of 48 and its karyotype formula was 48t, NF=48, and sex chromosome was not observed; the constitutive heterochromatin was observed at the centromeric region on most chromosomes; but only a single pair of NORs was detected at the paracentromeric region of chromosome pair No.24. Based upon above results, it is concluded that E. bleekeri was relatively primitive among genus Epinephelus.关键词
布氏石斑鱼/染色体/核型/银染/C-带Key words
Epinephelus bleekeri/ karyotype/ Ag-NORs/ C-bands分类
生物科学引用本文复制引用
蔡岩,周永灿,谢瑞敏,谢珍玉,冯永勤,王世锋..布氏石斑鱼的染色体核型、银染和C-带[J].水产学报,2012,36(5):647-651,5.基金项目
国家自然科学基金项目(31060355) （31060355）
农业科技成果转化项目(2009GB2E200302) （2009GB2E200302）
海洋渔业科学与技术浙江省重中之重学科开放课题资助项目(20100115) （20100115）
海南大学科研启动基金资助项目(kyqd1017) （kyqd1017）
海南大学"211工程"研究生科技创新平台建设项目 （）
