四川医学2012，Vol.33Issue(7)：1123-1126,4.
丙戊酸钠在体内外影响NK细胞对人肺癌细胞杀伤作用的研究
The effect of valproic acid the NK killing effects to the human lung cancer cell line in vitro and in vivo
仇滔 1芮军 1王友于 1龙丹 2周静2
作者信息
- 1. 成都市第三人民医院胸外科,四川,成都,610031
- 2. 四川大学华西医院移植免疫实验室,临检血液室,四川,成都,610041
- 折叠
摘要
Abstract
To investigate the influence of valproic acid ( VPA) in MICA gene expression in human lung carcinoma cells; A549,SP-CA-l,NCIH446,the infulence of valproic acid ( VPA) in NK cell's killing effect in lung carcinoma ceU and the difference of weight, size of tumor tissue in lung cancer tumor-burdened mice animal models. Methods 0. 75 ~ 12. 0mmol/ L of VPA were applied to treat A549 ,SP-CA-1 、NCIH446 cells. We observed the infulence of the VPA in the growth of lung carcinoma cells, compared to the untreated cells as the control group. RT-PCR was used to detect the changes of mRNA of MICA gene in three kinds of human lung carcinoma cells treated with 1. 5mmol/L and 3. 00mmol/L VPA for 4 days. The method of LDH was used to detect the difference of NK cells' kiUing effect in lung carcinoma cells before and after the treatment of VPA. Lung cancer tumor-burdened mice models was established to investigate the infulence of the VPA in weight, size of tumor tissue. Results The VPA inhibited the growth of three kinds of lung cancer cells above the concentration of 6. 0 mmol/L. Compared to the control group, the transcription level of mRNA of MICA gene were increased in the three kinds of human lung carcinoma cells treated with 1. 5mmol/L and 3. 00mmol/L VPA for 4 days. Difference was statistically significant for P < 0. 05. Higher degree of NK cells'killing effect in lung cancer cells which was inducted to express MICA by 1. 50mmol/L and 3. OOmmol/L VPA was observed, Compared to the control group. Difference was statistically significant for P < 0. 05. High VPA concentration group of lung cancer cells' mRNA and the extent of the damage by NK cells were higher than low concentration group. Difference was statistically significant for P < 0. 05. The inhibiting effect in the growth of tumor-burdened rat's subcutaneous tumor was significant in high VPA dose and/or low dose group, compared to the control group. Difference was statistically significant for P < 0. 05. The rates of inhibit of human lung cancer was 52. 9% in high VPA dose group and 35. 9% in low VPA dose group. Conclusion VPA induce theexpression of MICA, NK killing effect in the VPA( + ) treatment lung cancer cells was significantly stronger than that of VPA(-) condition. VPA also can inhibit the increase of lung cancer cell A549 in vivo. Also it implied a new mechanism of anti-cancer meth-od and new approach to cancer therapy.关键词
丙戊酸钠/肺癌细胞/MHC I类链相关基因A/NK细胞/小鼠Key words
valproic acid/ lung carcinoma cell/ mica/ nk cells/ mice分类
医药卫生引用本文复制引用
仇滔,芮军,王友于,龙丹,周静..丙戊酸钠在体内外影响NK细胞对人肺癌细胞杀伤作用的研究[J].四川医学,2012,33(7):1123-1126,4.
