山东科学2012，Vol.25Issue(3)：71-74,4.
纤维素酶法提取玫瑰花渣多糖工艺研究
Research on cellulase degradation extraction of polysaccharide in rose dregs
张曰辉 1马耀宏 2史建国 2杨俊慧2
作者信息
- 1. 山东轻工业学院,山东济南250353
- 2. 山东省科学院生物研究所,山东省生物传感器重点实验室,山东济南250014
- 折叠
摘要
Abstract
We employ single factor test and orthogonal test design method L9 （34） to analyze such influence factors as enzymolysis temperature, enzymolysis pH, enzymolysis time and cellulose amount on the yield rate of the polysaccharide. We also use reducing sugar analyzer to determine the amount of polysaccharide. Results show that temperature and pH are two dominant factors. The optimal enzymolysis condition is temperature of 50 ℃, pH of 4.6, 150 min and cellulose amount of 4.5%. The extract ratio of rose dregs polysaccharide is 11.1% and the yield rate of soluble polysaccharide is 4.49% in condition of the ratio of rose dreas and water of 1： 30. temoerature of 100 ℃ and extract time of 5 h.关键词
玫瑰花渣/多糖/纤维素酶Key words
rose dregs polysaccharide cellulase分类
医药卫生引用本文复制引用
张曰辉,马耀宏,史建国,杨俊慧..纤维素酶法提取玫瑰花渣多糖工艺研究[J].山东科学,2012,25(3):71-74,4.
