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基于DNS原理的还原糖测定仪

高广恒 周万里 朱思荣 毕春元 赵晓华 史建国

山东科学2012,Vol.25Issue(3):95-97,3.
山东科学2012,Vol.25Issue(3):95-97,3.

基于DNS原理的还原糖测定仪

A DNS based reducing sugar detector

高广恒 1周万里 2朱思荣 2毕春元 2赵晓华 2史建国2

作者信息

  • 1. 山东省科学院中日友好生物技术研究中心,山东省生物传感器重点实验室,山东济南250014
  • 2. 山东省科学院生物研究所,山东济南250014
  • 折叠

摘要

Abstract

We designed a DNS based reducing sugar detector. It has such advantages as low-concentration reducing sugar detection, convenient operation, easy maintenance, high accuracy and good repeatability. It employs colorimetry to detect the differential light absorption value of dye liquor before and after heating and then to calculate the content of reducing sugar in a sample. We discover that the content of reducing sugar is proportional to the light absorption value. Glucose of 0.1% is used to calibrate measurement. The linear rate of more than 0.99 exists when the concentration range is between 0% and 0.1%. The detector can stably detect the content of reducing sugar in beer, sorbitol and ferment.

关键词

DNS/还原糖/比色法

Key words

DNS/reducing sugar/colorimetry

分类

信息技术与安全科学

引用本文复制引用

高广恒,周万里,朱思荣,毕春元,赵晓华,史建国..基于DNS原理的还原糖测定仪[J].山东科学,2012,25(3):95-97,3.

山东科学

OACSTPCD

1002-4026

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