山东医药2012，Vol.52Issue(16)：1-3,6,4.
GST-hPlk2融合蛋白表达载体的构建及其在原核细胞中的表达
Construction of GST-hPlk2 fusion protein expression vectors and the expression in prokaryotic cells
摘要
Abstract
Objective To construct GST-hPlk2 fusion protein expression vector and induce its expression in Esche-richia coli (E. coli). Methods Total mRNA was extracted from HEK293 cells, and cDNA was formed by reverse transcription. The hPlk2 coding sequence was amplified by polymerase chain reaction (PCR) and subcloned into pGEX-4T-2 vector. The positive recombinant was identified by restriction enzyme digestion and DNA sequencing. Then they were transformed into E. coli BL21, induced by IPTC and identified by SDS-PAGE and Western blot. Results The prokaryotic expression plasmid pGEX-4T-2-hPlk2 was successfully constructed and confirmed by enzyme digestion and sequencing. The CST-hPlk2 fusion proteins were expressed and confirmed by Western blot. Conclusions The prokaryotic expression plasmid of hPlk2 was successfully constructed and the expression of fusion proteins in E. coli was confirmed. This study provides the basis for the further research on purifying Plk2 protein and the biological function of Plk2.关键词
hPlk2/原核细胞/表达/融合蛋白Key words
hPlk2/ prokaryotic cells/ expression/ fusion proteins分类
医药卫生引用本文复制引用
沈涛,杨礼庆,李妍,梁峰,梁爽,付勤..GST-hPlk2融合蛋白表达载体的构建及其在原核细胞中的表达[J].山东医药,2012,52(16):1-3,6,4.基金项目
国家自然科学基金资助项目(30800415) （30800415）
辽宁省科技计划项目(2009225010-15). （2009225010-15）
