色谱2012，Vol.30Issue(4)：374-377,4.DOI:10.3724/SP.J.1123.2011.12064
在线样品前处理大体积进样离子色谱法直接测定海水中亚硝酸盐、硝酸盐和磷酸盐
Direct determination of nitrites, nitrates and phosphates in seawater using suppressed conductivity ion chromatography with large volume injection and on-line sample preparation techniques
摘要
Abstract
Using on-line pre-chlorine and sulfate removal technique, a rapid and direct method was established for the simultaneous determination of nitrite-N, nitrate-N and phosphate-P in seawater by ion chromatography (IC) with suppressed conductivity detection. In this method, an lonPac AG23 column was used as the enrichment column, an IonPac AS23 column with high capacity as the analytical column, and KOH solution as the eluent for gradient elution produced by an eluent generator on-line automatically. The results showed that the determination of the targets was not interfered when a seawater sample diluted 5 to 10 times was directly injected. The method detection limits (MDL) of nitrite-N, nitrate-N and phosphate-P were 0. 3, 0.4 and 0.2μg/L respectively, under the conditions of 500μL sample volume and 1 mL/rain elution rate. The linear ranges of nitrite-N, nitrate-N and phosphate-P were 10 -500 μg/L, 14 -680 μg/L and 3.4 -170μg/L respectively, and the correlation coefficients (r) were all greater than 0.999 0. The average recoveries of the 3 analytes spiked in a sample ranged from 92% to 106% with the relative standard deviations (RSDs,n = 6) of 1.2% -7. 7%. The whole sample analysis process can be completed in 13 min. The method has the advantage of simple operation, reagent-free and pollution-free, and can meet the quantitative analysis requirements of seawater.关键词
在线样品前处理技术/大体积进样/离子色谱/亚硝酸盐/硝酸盐/磷酸盐/海水Key words
on-line sample preparation technique/ large volume injection/ ion chromatography (IC) / nitrites/ nitrates/ phosphates/ seawater分类
化学化工引用本文复制引用
林红梅,林奇,张远辉,张静静,陈立奇..在线样品前处理大体积进样离子色谱法直接测定海水中亚硝酸盐、硝酸盐和磷酸盐[J].色谱,2012,30(4):374-377,4.基金项目
国家海洋局第三海洋研究所基本科研业务费专项资金项目(海三科2008002)和中国极地科学战略研究基金项目(20080222). （海三科2008002）
