色谱2012，Vol.30Issue(5)：487-494,8.DOI:10.3724/SP.J.1123.2011.12038
新型亲水整体柱的制备及其在毛细管液相色谱和加压电色谱中的应用
Preparation of new hydrophilic monolithic columns and their applications in capillary liquid chromatography and pressurized capillary electrochromatography
摘要
Abstract
New hydrophilic monolithic columns were prepared with | [3-( methacryloylamino) propyl] dimethyl(3-sulfopropyl) ammonium hydroxide inner salt (SPP) | as monomer, pentae-rythritol triacrylate (PETA) as crosslinking agent, azobisisobutyronitrile (AIBN) as initiator with two different porogens consisting of ethanol/ethylene glycol and methanol/1,4-butanediol, separately. In order to obtain monolithic columns with satisfactory efficiency, electroosmotic flow (EOF) velocity and permeability, the contents of the polymerization mixture were investigated and optimized. The performances of the two columns were compared in the permeability and separation performance. It was found that the monolithic column prepared with ethanol/ ethylene glycol had a better column efficiency and selectivity than that with methanol/1,4-butanediol, but was inferior to the latter in permeability. The effect of salt concentration ( from 10 to 70 mmol/L ammonium formate) on the retention of nucleosides was investigated. It was observed that the retention factors of these nucleosides increased at first and then decreased. The columns were used in capillary liquid chromatography (cLC) and pressurized capillary electrochromatography (pCEC) for the separation of a test mixture of amines, phenols and nucleosides separately, and satisfactory separations for these samples were achieved. The column used in pCEC system showed better separation and higher speed of the mixture consisted of phenols and nucleosides compared to those used in cLC system.关键词
亲水整体柱/制备/毛细管液相色谱/加压毛细管电色谱/胺类化合物/酚类化合物/核苷类化合物Key words
hydrophilic monolithic column/ preparation/ capillary liquid chromatography(cLC)/ pressurized capillary electrochromatography (pCEC)/ amines/ phenols/ nucleosides分类
化学化工引用本文复制引用
高也,王彦,王超然,谷雪,阎超..新型亲水整体柱的制备及其在毛细管液相色谱和加压电色谱中的应用[J].色谱,2012,30(5):487-494,8.基金项目
国家自然科学基金项目(21175092,21105064)和国家重大科学仪器设备开发专项(2011YQ150072,2011YQ15007204,2011YQ15007207和2011YQ15007210). （21175092,21105064）
