生物加工过程2012，Vol.10Issue(3)：1-5,5.DOI:10.3969/j.issn.1672-3678.2012.03.001
基于辅因子调控对大肠杆菌两阶段发酵产丁二酸的影响
Effect of cofactor regulation on succinate production in Escherichia coli during dual-phase fermentation
摘要
Abstract
Two strains, including E. coli NZN111 and its recombinant strain E. coli NZNlll/pTrc99a-pncB were investigated as the production of succinate. During dual-phase fermentation, E. coli NZN111 generated succinate as the main products, meanwhile, pyruvate was accumulated. With the overex-pressed the nicotinic acid phosphoribosyl transferase, the recombinant strain lowered the concerntration of pyruvate, eliminated the generation of acetate and increased the production of succinate under dual-phase fermentation. The yield of succinate and the glucose consumption rate was increased by 139% , and 20% , respectively.关键词
大肠杆菌/烟酸/转磷酸核糖激酶/丁二酸Key words
E. coli/ nicotinic acid/ phosphoribosyl transferase/ succinate分类
生物科学引用本文复制引用
刘嵘明,梁丽亚,王光明,张敏,马江锋,陈可泉,姜岷,韦萍..基于辅因子调控对大肠杆菌两阶段发酵产丁二酸的影响[J].生物加工过程,2012,10(3):1-5,5.基金项目
国家自然科学基金资助项目(21076105) （21076105）
国家重点基础研究发展计划(973计划)资助项目(2009CB724701) （973计划）
