生物加工过程2012，Vol.10Issue(3)：28-32,5.DOI:10.3969/j.issn.1672-3678.2012.03.006
产紫青霉中β-葡萄糖醛酸苷酶的诱导表达
Induction of β-glucuronidase in Penicillium purpurogenum Li-3
摘要
Abstract
Carbon catabolite repression was found when Penicillium purpurogenum Li-3 grew in medium containing other carbon sources than glycyrrhizin. The induction strategy of β-glucuronidase was studied and a new technology for the enzyme producing induction was established, which based on the optimization of fermentation conditions. The inducer was added after obtaining a large mycelium biomass in nutrient medium with glucose as the sole carbon source. When glucose was completely consumed in the nutrient medium containing 5 g/L of glucose, 20% inducer (10% glycyrrhizin + 1. 2% Tween80) was added every 24 h. Then the culture temperature was moved to 40 ℃ before fermentation lasted for 48 h. The phenomenon of " second production" of P. purpurogenum Li-3 was found after the procedure. The enzyme activity was enhanced from 647. 99 U/mL to 2 356 U/mL, thus it raised about three fold.关键词
C代谢/β-葡萄糖醛酸苷酶/诱导/产紫青霉Key words
carbon catabolite/β-glucuronidase/ induction/ Penicillium pururogenum分类
化学化工引用本文复制引用
郭晓晓,王小艳,冯世江,李春..产紫青霉中β-葡萄糖醛酸苷酶的诱导表达[J].生物加工过程,2012,10(3):28-32,5.基金项目
国家自然科学基金资助项目(20976014,21176028) （20976014,21176028）
教育部博士点基金资助项目(20091101110036) （20091101110036）
北京市自然科学基金资助项目(2112035) （2112035）
北京理工大学基础研究基金资助项目(2011cx010) （2011cx010）
