生物加工过程2012，Vol.10Issue(3)：50-55,6.DOI:10.3969/j.issn.1672-3678.2012.03.010
纳米银复合介孔碳材料制备固定化猪胰脂肪酶
Porcine pancreatic lipase immobilised on nano-silver mesoporous activated carbon composite
摘要
Abstract
The study was carried out to immobilize porcine pancreatic lipase(PPL) on nano-silver mesoporous activated carbon(Ag-MAC). MAC was prepared from rice husk by a two stage process and nano-silver was absorbed on the surface. PPL was then immobilized on Ag-MAC and conditions were optimized. Results showed that under the conditions of the silver nitrate concentration of 0. 01 mol/L, immobilization time of 6 h, reactive phosphate solution of pH 6.0, reaction temperature 25 ℃, the lipase activity increased by 3.2 times. Meanwhile, N2 adsorption-desorption( BET) , scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy(FT-IR) were used to analyze experimental results, and confirm the effects of Ag-MAC in experiments of lipase immobilization.关键词
介孔碳材料/固定化/纳米银/猪胰脂肪酶Key words
mesoporous activated carbon/ immobilization/ nano-silver/ porcine pancreatic lipase分类
生物科学引用本文复制引用
杨波,王伟,朱凯,王欢,韩萍芳..纳米银复合介孔碳材料制备固定化猪胰脂肪酶[J].生物加工过程,2012,10(3):50-55,6.基金项目
国家重点基础研究发展计划(973计划)资助项目(2009CB72470) （973计划）
