生物加工过程2012，Vol.10Issue(3)：56-60,5.DOI:10.3969/j.issn.1672-3678.2012.03.011
桑黄粗多糖除蛋白及抗肿瘤活性
Deproteinization and antitumor activity of Phellinus igniarius crude polysaccharides
摘要
Abstract
The optimum technology of Sevag method was studied to remove protein from Phellinus igniarius crude polysaccharides, and test antitumor activity of polysaccharides by MTT method. On the basis of single factor experiment, including the amount of Sevag reagent,the volume ratio of chloroform and buta-nol,extraction times and shaking time, an orthogonal experiment was designed. After analysis of variances, the optimum technology of Sevag method was determined. Then, anti-tumor experiment in vitro was tested. The results showed that the optimum technology conditions of Sevag method were as follows the amount of Sevag was 50 mL, the volume ratio of chloroform to butanol was 3, the extraction times was 6, and the shaking time was 20 min. The result of cell experiment in vitro showed that the inhibition toward cancer cell EC 109 and SiHa between the crude and deproteinizative polysaccharides had no significant difference.关键词
桑黄/多糖/Sevag法Key words
Phellinus linteus/ polysaccharides/ Sevag method分类
生物科学引用本文复制引用
梁大勇,黄芳,赵晨,于朋,秦丹,宋爱荣..桑黄粗多糖除蛋白及抗肿瘤活性[J].生物加工过程,2012,10(3):56-60,5.基金项目
山东省科技攻关资助项目(2010GNC10956) （2010GNC10956）
