生物信息学2012，Vol.10Issue(2)：79-82,4.DOI:10.3969/j.issn.1672-5565.2012.02.03
X病毒属6个成员中国分离物CP基因的克隆及同源性分析
Cloning and phylogenetic analysis of six allexivirus CP genes from China isolates
摘要
Abstract
Specific primers were designed to amplify CP genes of Garlic virus A, Garlic virus B, Garlic virus C, Garlic virus D, Garlic virus E and Garlic virus X China isolates, their multiple alignment and phylogenetic analysis were made. The results indicated that CP genes of Garlic virus A, Garlic virus B , Garlic virus C, Garlic virus D , Garlic virus E and Garlic virus X consists of 756, 735, 780, 753, 759 and 732 nucleotide acids respectively. Multiple alignment shows that GarVC and GarVD shared the lowest amino acids identities (57.69% ) and GarVB and GarVX shared the highest amino acids identities (74. 71% ) among six CP genes. Phylogenetic analysis shows that GarV - A, GarV - E and GarV - D has closer relationship among them, GarV - B and GarV - X has closer relationship with each other and GarV - C has remoter relationship with other five CP genes. These results would shed light on predetermination of serology relationship among six kinds of Allexivirus virus and predetermination of the interference among them by ELISA analysis.关键词
葱属X病毒属/CP基因/基因克隆/同源性分析/生物信息学Key words
Allexivirus/ CP gene/ Gene cloning/ Phylogenetic analysis/ Bioinformatics分类
生物科学引用本文复制引用
隗洋洋,韦传宝,杨宇,华俊雅..X病毒属6个成员中国分离物CP基因的克隆及同源性分析[J].生物信息学,2012,10(2):79-82,4.基金项目
安徽省教育厅自然科学基金重点项目资助(KJ2011A272,KJ2010A328). （KJ2011A272,KJ2010A328）
