生物信息学2012，Vol.10Issue(2)：96-100,5.DOI:10.3969/j.issn.1672-5565.2012.02.08
芦笋EST资源的SSR信息分析
Analysis of SSR information in EST resource of asparagus
摘要
Abstract
To develop asparagus EST -SSRs function of molecular marker, Simple sequence repeats!(SSRs) were investigated in 8590 qualified ESTs downloaded from Nucleotide ESTs database of Lentinula edodes in NCBI . After eliminated redundancy, we got 8377 sequence of nonredundancy, in which there were 469 EST - SSRs, the mean distance was 14.80 kb in non - redundant ESTs. Of the total EST - SSRs, 40.51% (190/469 ) were dinucleotides repeats(DNRs) ,34.79% (164/469) were trinucleotide repeats (TNRs) and 21.11% (99/469) were hexanucle-otide repeats (HNRs). In which the repeat motifs CT/AG were the most abundant up to 13. 22% (62/469). Random filter out 30 EST - SSR sequences, then using primer5 software designed its primers for PCR amplification. 27 of primers showed amplifications, in which 21 pairs could amplify distinct bands ,accounted for 80% (24/30). Al-leles in asparagus were abundant , average 4. 93 each pair. These EST - SSR markers will be useful in studies of population genetics, linkage mapping, molecular mapping, gene tagging, and pedigree analysis and other aspects for this species.关键词
芦笋/EST -SSRs/引物Key words
Asparagus/ EST - SSRs / primers分类
农业科技引用本文复制引用
吴建霞,李亚玲,张智俊,管雨..芦笋EST资源的SSR信息分析[J].生物信息学,2012,10(2):96-100,5.基金项目
国家“863”项目(2007AA021403) （2007AA021403）
浙江省自然科学基金资助项目(Y307469) （Y307469）
浙江农林大学人才启动基金. （）
