山西大学学报(自然科学版)2012,Vol.35Issue(2):383-387,5.
飞蝗两种群羧酸酯酶及谷胱甘肽S-转移酶活性与马拉硫磷敏感性关系的研究
Activities of Carboxylesterases and Glutathione S-transferases Related to Malathion Susceptibility in two Populations of Locusta migratoria
摘要
Abstract
A series of comparative analysis including bioassay, biochemical studies to the locust collected from Tianjin beidagang and Hebei cangzhou were conducted. The bioassay indicated that Tianjin Beidagang population was tolerance to malathion (tolerance ratio=13. 2). The carboxylesterases (CarEs) specific activity with σ-naphthyl butyrate (σ-NB) and β-naphthyl acetate (β-NA) substrates of the Hebei Cangzhou population was significantly higher compared with Tianjin Beidagang population. While the a-naphthyl acetate (crNA) was used as substrate, the significant difference of CarEs specific activity between two populations was not detected. The specific activity of glutathione S-transferases (GSTs) of Hebei Cangzhou population was significantly higher than these of Tianjin Beidagang population when l-chloro-2,4-dinitroben-zene (CDNB) was used as substrate. All these results suggested that the observed tolerance to malathion in the Tianjin Beidagang population was not conferred by the CarEs and GSTs,but possibly related to the reduction of the target sensitivity.关键词
飞蝗/羧酸酯酶/谷胱甘肽S-转移酶/马拉硫磷Key words
Locusta migratoria /Carboxylesterase/Glutathione S-transferases/malathion分类
生物科学引用本文复制引用
马恩波,葛娉婷,张建琴,张建珍..飞蝗两种群羧酸酯酶及谷胱甘肽S-转移酶活性与马拉硫磷敏感性关系的研究[J].山西大学学报(自然科学版),2012,35(2):383-387,5.基金项目
国家自然科学基金重大国际合作项目(30810103907) (30810103907)
国家自然科学基金(30870302) (30870302)
山西省自然科学基金(2011011033-1) (2011011033-1)
公益性农业科研项目(200903021) (200903021)