陕西师范大学学报：自然科学版2012，Vol.40Issue(3)：71-75,5.
人泡沫病毒GAG、ENV蛋白的原核表达、抗血清制备及鉴定
Prokaryotic expression, purification, and antiserum preparation of GAG and ENV proteins of human foamy virus
摘要
Abstract
In order to detect the efficiency of human foamy virus infection on protein expression level, the recombinant prokaryotic expression vectors pET-32a-GAG and pET-32a-ENV were constructed successfully and transformed into E. coli BL21 Star （DE3）, and the GAG, ENV fusion proteins were induced by IPTG and expressed at high level. Subsequently, the New Zealand rabbits were immunized with the fusion proteins for preparing the antiserums. Western Blot analysis showed that the antiserum could interact with GAG and ENV fusion proteins specifically. Based on the results from Western Blot and indirect immunofluotesent method, it is concluded that the antiserums were verified to interact with the natural GAG and ENV expressed from the viruses.关键词
人泡沫病毒/GAG蛋白/ENV蛋白/原核表达/抗血清Key words
human foamy virus/GAG protein/ENV protein/prokaryotie expression/antiserum分类
医药卫生引用本文复制引用
卢飞,孙燕,李晶,李治..人泡沫病毒GAG、ENV蛋白的原核表达、抗血清制备及鉴定[J].陕西师范大学学报：自然科学版,2012,40(3):71-75,5.基金项目
基金项目：教育部科学技术研究重点项目 （）
国家自然科学基金资助项目（31000086）. （）
