山西医科大学学报2012，Vol.43Issue(4)：253-256,318,5.DOI:10.3969/J.ISSN.1007-6611.2012.04.004
地塞米松对M-CSF体外诱导分化的骨髓巨噬细胞的影响
Influence of dexamethasone on M-CSF-induced bone marrow macrophage in vitro
王欢 1付丽 2王昭 2熊梅1
作者信息
- 1. 首都医科大学附属北京友谊医院医疗保健中心,北京100050
- 2. 首都医科大学附属北京友谊医院血液内科
- 折叠
摘要
Abstract
Objective To explore the regulatory effects of dexamethasone on bone marrow macrophage cultured by M-CSF in vitro. Methods M-CSF-induced bone marrow macrophage were cultured with the different concentrations of dexamethason.and the morphology and the cell proliferation were observed. Immunofluorescence method was performed to examine the phagocytic function,and RT-PCR technique was used to measure the mRNA levels of inflammatory factors. Results Proliferation of the cultured cells was inhibited by dexamethasone in a dose-dependent manner. The phagocytic functions of M-CSF-induced bone marrow macrophage were effectively inhibited by dexamethasone. The cultured cells were oval like,and their pseudopod became wide and short. Dexamethasone inhibited the related pro-inflammatory factors in the cultured cells. Conclusion The results suggest that dexamethasone could inhibit the activity and function of M-CSF-induced bone marrow macrophage in vitro,which provide an experimental basis in the treatment of hemophago-cytic lymphohistioeytosis.关键词
地塞米松/巨噬细胞/吞噬作用Key words
dexamethasone/macrophage/phagocytosis分类
医药卫生引用本文复制引用
王欢,付丽,王昭,熊梅..地塞米松对M-CSF体外诱导分化的骨髓巨噬细胞的影响[J].山西医科大学学报,2012,43(4):253-256,318,5.
