陕西医学杂志2012，Vol.41Issue(7)：775-777,779,4.
黄芩苷对白血病细胞耐药株逆转耐药效果及机制研究
Reversal effect and machenism induced by baicalin on leukemia cell line
杨莉洁 1赵挺 1白庆咸 1董红娟1
作者信息
- 1. 第四军医大学西京医院血液科,西安710032
- 折叠
摘要
Abstract
Objective: To observe reversal effect of baicalin on the leukemia cells, and to explore its possible mechanism. Methods: The inhibition rates of adriamycin and baicalin on parental cells or drug-resistant leukemia cells were determined by MTT assay, evaluation of multiple drug resistance and the reversal effect of baicalin was calculated according to half inhibitory concentration (IC50) of adriamycin on adriamycin-resistant cell lines and parental cells, effect of baicalin on drug accumulation of adriamycin in drug-resistant cells was analysed by flow cytometry assay, RT-PCR detection was executed to investigate MDR1, MRP1 and LRP1 expression in the resistant cells after treatment of baicalin. Results: The Resistance Index of drug-resistant cell lines was 17. 384, the reverse rate of baicalin with 10μg/ml and 20μg/ml were 4. 230 and 7. 812, respectively, relative reversal rate were 0. 81 and 0. 93 , baicalin could significantly increase adriamycin accumulation and decrease the expression of MDR1 gene significantly. Conclusion; Baicalin illustrated a significant reversal effect on the leukemia cell line, the mechanism may be to reduce MDR1 gene expression in the cell to keep p-glycoprotein pumping the adriamycin out of cells.关键词
黄芩苷/白血病/多药耐药相关蛋白质类/基因表达Key words
Baicalin/Leukemia/Multidrug resistance-associated proteins/Gene expression分类
医药卫生引用本文复制引用
杨莉洁,赵挺,白庆咸,董红娟..黄芩苷对白血病细胞耐药株逆转耐药效果及机制研究[J].陕西医学杂志,2012,41(7):775-777,779,4.
