陕西医学杂志2012，Vol.41Issue(7)：780-783,4.
急性胰腺炎不同病理类型基因表达谱差异的研究
Study of gene expression profile disparity in different pathology types of acute pancreatitis
摘要
Abstract
Objectives: To study the difference of gene expression profile changes in normal pancreas、 MAP and SAP tissues by cDNA microarray and to explore the molecular mechanisms in the development of AP. Methods; The cDNA retro-transcribed from equal quantity mRNA from normal pancreas、 MAP and SAP tissues which were labeled with Cy5 and Cy3 fluorescence as probes. The mixed probe was hybridized with three pieces Bio-starH-40s double dot human whole gene chip. The chips were scanned with a ScanArray 4000. The acquired images were analyzed by using GenePix Pro 3. 0 software. Results: A total of 141 genes were screened out that exhibited differentially expression in all three chips. It was identified that in gene expression profiles of MAP there were more than 2 times include 74 upregulated and 67 downregulated genes compared with SAP. Conclusions: A comparison between this two gene profiles shows that; The gene expression level is different in SAP and MAP epithelium. It is shown that these 141 genes probably relate to the occurrence and development of SAP and to the promotion or progress in MAP.关键词
胰腺炎,急性坏死性/病理学/DNA微阵列/基因表达谱Key words
Pancreatitis , Acute necrotizing/pathology/@DNA microarrays/Gene expression profiling分类
医药卫生引用本文复制引用
刘斌,刘瑞廷,管来顺,常英,韩希望..急性胰腺炎不同病理类型基因表达谱差异的研究[J].陕西医学杂志,2012,41(7):780-783,4.基金项目
陕西省科技计划社会发展攻关项目[2005 K13-G6(11)] （11）
