实用妇产科杂志2012，Vol.28Issue(5)：368-371,4.
妊娠期肝内胆汁淤积症孕妇胎盘合体滋养细胞OATP8基因表达及意义的研究
Expression and Significance Study of Organic Anion Transporting Polypeptides8 Gene in Placenta Syntrophoblastic Cell of Intrahepatic Cholestasis of Pregnancy
摘要
Abstract
Objective:To explore the expression and significance of organic anion transporting polypep-tides 8 gene(OATPS) in placenta syncytiotrophoblast of intrahepatic cholestasis of pregnancy(ICP). Methods: Patients were divided into three groups: mild ICP group(8 cases), severe ICP group(8 cases) and control group(8 cases),The location of OATP8 protein in placenta was checkedby Immunohistochemical SABC method. OATP8 mRNA and protein levels were determined by reverse transcription polymerase chain reaction (RT-PCR) and western blot. Results :①OATP8 protein was located on the cell membrane of placenta syncytiotrophoblast. ②The mRNA expression of OATP8 in severe ICP group was higher than that in control and mild ICP group(P<0.05). The mRNA expression of OATP8 in mild ICP group was higher than that in control group, were OATP8 in severe ICP group(P<0.05). ③OATP8 protein expression in the severe ICP group was significantly higher than that in mild group (P < 0.05), and the protein in mild group was higher than that in control group ( P<0.05). Conclusions:OATP8 protein expression in placenta syncytiotrophoblast increases appreciably in ICP pregnant women, which may contribute to the scavenging process of bilirubin or bileacid.关键词
妊娠期肝内胆汁淤积症/有机阴离子转运多肽8/胆红素/胎盘Key words
Intrahepatic cholestasis of pregnancy/ Organic anion transporting polypeptide 8/ Bilirubin/Placenta分类
医药卫生引用本文复制引用
李秋红,邵勇..妊娠期肝内胆汁淤积症孕妇胎盘合体滋养细胞OATP8基因表达及意义的研究[J].实用妇产科杂志,2012,28(5):368-371,4.基金项目
重庆市科委自然科学基金(编号:CSTC2007BB5278) （编号:CSTC2007BB5278）
重庆市卫生局基金(编号:06-2060 （）
2009-2386) （）
