实用肝脏病杂志2012，Vol.15Issue(4)：321-323,3.DOI:10.3969/j.issn.1672-5069.2012.04.017
慢性丙型肝炎患者病毒基因分型与血清HCV RNA水平的关系探讨
Serum HCV RNA levels in chronic hepatitis C patients with different genotypes
摘要
Abstract
Objective To determine the distribution of hepatitis C virus genotypes in patients with chronic hepatitis C, and to study the serum HCV RNA levels in patients with different HCV genotype infection. Methods The levels of serum HCV RNA was measured with real -time fluorescent quantitative reverse -transcription polymerase chain reaction,the serum anti -HCV was detected by ELISA,ALT by automatic biochemistry analyzer, the levels of hyaluronic acid, laminin, procollagen type M N-terminal peptide and collage type IV were detectd by chemiluminescence iummunoassay and the HCV genotypes in 218 patients with chronic hepatitis C were determined by gene chip. Results There were nine different subgenotypes of HCV in 218 samples, including genotype lb,2a,3a,3b and 6 genotype in 208 sera and lb+2a, lb+3b, lb+6,2a+3a in other 10 sera, respectively; Genotype lb was the main one (77.1%, 168/218) , and the genotype 2a was the second (8.7%, 19/ 218); There was no significant difference between the serum HCV RNA levels in 208 patients with different HCV genotype infection (F =0.932, P>0.05); There was no significant difference between HCV genotype and gender as well as the levels of four blood markers of hepatic fibrosis in 168 patients with lb and 40 with non-genotype lb infection (x2=0.857,P>0.05). Conclusions HCV lb is the main prevalent genotype in patients with chronic hepatitis C, and the serum level of HCV RNA is not related to HCV genotype infection.关键词
慢性丙型肝炎/HCV RNA/基因芯片/基因分型Key words
Hepatitis C/ Real-time fluorescent quantitative PCR/Gene chip/Genotypes引用本文复制引用
常静霞,张怡青,王洁,孟运运,汪茂荣..慢性丙型肝炎患者病毒基因分型与血清HCV RNA水平的关系探讨[J].实用肝脏病杂志,2012,15(4):321-323,3.基金项目
国家十一五科技重大专项课题(编号:2008ZX10002-014) （编号:2008ZX10002-014）
