实用肝脏病杂志2012，Vol.15Issue(4)：332-335,4.DOI:10.3969/j.issn.1672-5069.2012.04.020
CXCR3基因敲除小鼠慢性乙型肝炎病毒复制模型的建立
Establishment of a hepatitis B viral infection model in CXC chemokine receptor 3- knocked-out mice
摘要
Abstract
Objective To establish a HBV infection model of CXCR3 knocked-out mice. Methods After CXCR3 knocked- out, nine of them and nine wild-type C57BL/6 mice were injected hydrodynamically ten micrcg-ams of pAAV/HBV1.2 DNA into the tail veins. Then, the mice were regularly bled to monitor the serum levels of HBsAg, HbeAg and HBV DNA. The HBcAg in the livers torn injected mice were detected by immunohistochemistry. Results All the bred CXCR3 knocked-out mice were homozygous. The serum levels of HBsAg from the CXCR3 knocked-out mice and the wild type C57BL/6 were 1134.69± 244.42 and 1759.63± 881.20 GM).O96) at the first day after the injection of the pAAVHBV1.2 piasmids; 5305.29 ± 1395.06 and 7493.29± 658.63 (P=0.003) at the fourth day, 1615.04± 1187.16 and 1536.19± 1046.02 (P=0.905) at the fifteenth day,and 229.45± 79.27 and 228.19± 295.02(P=0.996) at fortieth day,respectively;the serum levels of HBeAg from them were 6.65± 1.50 and 20.61 ± 4.03 (P=0.000) at the first day,6.33± 1.61 and 9.79± 2.31 (P=0.007) at the fourth day,3.52± 1.97 and 2.85± 0.74 (P=0.425) at the fifteenth day,and 1.28± 0.06 and 1.90± 1.01 (P=0.431)at fortieth day, respectively and the serum levels of HBV DNA from them were 4.38± 0.22 Igcopies/ml and 6.56± 0.16 Igcopies/ml (P=0.008) at day10,4.41± 0.88 Ig copies/ml and 5.69± 0.04 Ig copies/ml(P=0.177) at day15,4.48± 0.04 Igcopies/ml and 6.44± 0.16 lgcopies/m(P=0.004) at day25,and 3.66± 0.45 Igcopies/ml and 5.20± 0.28 Ig copies/ml (P=0.055) at day40,respectively;HbsAg,HbeAg and HBV DNA in CXCR3 knocked-out mice were continuously positive untill the day 40 after the injection of the pAAV/HBV1.2 DNA;Both cytoplasmic and nuclear HBcAg were detected in the livers of the CXCR3 knocked-out mice at the day 4,15,40 after the infection. The positive Serum HBsAg, HbeAg and HBV DNA in CXCR3 knocked- out mice were similar to those in the wild type C57BIV6 mice. Conclusion We had successfiilly established a HBV infection model in CXCR3 knocked-out mouse, which might be helpfiil way to investigate the relationship between the CXCR3 and its ligands to the HBV infection.关键词
慢性乙型肝炎/CXCR3基因敲除小鼠/CXCR3/趋化因子Key words
Hepatitis B/CXCR3 knocked-out mice/CXCR3/Chemokine引用本文复制引用
陈明发,吴珺,夏幼辰,林永,孙潺,杨东亮..CXCR3基因敲除小鼠慢性乙型肝炎病毒复制模型的建立[J].实用肝脏病杂志,2012,15(4):332-335,4.基金项目
国家自然科学基金(No.81001313) （No.81001313）
中国博士后基金(No.20090460949) （No.20090460949）
