实用肝脏病杂志2012，Vol.15Issue(4)：343-345,3.DOI:10.3969/j.issn.1672-5069.2012.04.023
黄芩苷诱导人肝癌HepG-2细胞凋亡作用观察
Inhibition of apoptosis of HepG-2 cells by bacalin in vitro
梁慧敏 1时小燕 1和振坤 1徐庆杰1
作者信息
- 1. 475001河南省开封市,河南大学淮河医院感染病科
- 折叠
摘要
Abstract
Objective To investigate the inhibition of apoptosis of HepG-2 cells by bacalin in vitro. Methods Human hepatocellular carcinoma cells, HepG-2 were cultured with different concentrations of baicalin and the inhibitory rate of cell proliferation was calculated with MTT assay; Morphology changes were detected by Hoechst33258/PI double fluorescence coloration; The apoptosis rate was detected by TUNEL;the Caspase-9, Caspase-3 and Bel-2 protein expression were detected by Western blot. Results The cell proliferation was inhibited by baicalin at concentration of 25 to 100u g/ml;the inhibitory rate in 48h was 50.63% at 50u g/ml and 77.62% at 75u g/ml,and the inhibitory effect was in the concentration and time-dependent manner;there was a typical apoptosis at 50u g/ml in 48h,showing cell shrinkage and fragmentation of cell nucleus;the caspase-9 and caspase-3 expression increased and Bel-2 decreased as the concentration of baicalin increased. Conclusion Baicalin can inhibit proliferation of HepG2 cells by inducing apoptosis and the mechanisms might be related to the mitochondrial pathway.关键词
肝癌/HepG2细胞/黄芩苷/细胞凋亡Key words
Hepatoma/ HepG2 cells/ Baicalin/ Apoptosis引用本文复制引用
梁慧敏,时小燕,和振坤,徐庆杰..黄芩苷诱导人肝癌HepG-2细胞凋亡作用观察[J].实用肝脏病杂志,2012,15(4):343-345,3.
