生殖医学杂志2012，Vol.21Issue(3)：252-256,5.DOI:10.3969/j.issn.1004-3845.2012.03.013
抗氧化剂半胱氨酸对小鼠卵母细胞体外成熟及发育潜能的影响
Effects of anti-oxidant cysteine on in vitro maturation and development potential of mouse oocytes
黄品秀 1李蓉 1林洪波 1刘波澜 1赵善飞1
作者信息
- 1. 北京大学深圳医院生殖医学中心,广东深圳518036
- 折叠
摘要
Abstract
Objective: To investigate the effects of anti-oxidant cysteine on in vitro maturation and development potential of mouse oocytes for further optimizing the system of oocytes matured in vitro.
Methods: One hundred and one GV oocytes of mouse were cultured in the in vitro culture based medium which consisted of HTF +0. 075 IU/ml HMG+0. 5 IU/ml hCG +1 μg/ml E2 in control group. One hundred and fifteen GV oocytes of mouse were cultured in the medium mentioned above plus 0. 6 mmol/L cysteine in the experimental group. The oocytes in both groups were cultured for 16-18 hours, and then the maturation rates, glutathione levels and mitochondrial membrane potential were observed.
Results.- The oocytes maturation rate in the experimental group 80. 0% (92/115) was significantly higher than that in the control group [68. 3% (69/101) ](P<0. 05). The average glutathione levels in the content of oocytes of the experimental group were 0. 51 ±0. 5 μg/L and slightly higher than those in the control group (0. 48i0. 6 μg/L) but there was no statistically significance (P>0. 05). The mitochondrial membrane potential in the experimental group was 0. 39 ±0. 02△Ψm and significantly higher than that in the control group (0. 04±0. 003△Ψm) (P<0. 05). Conclusions; Anti-oxidant cysteine played a significant protective effect on in vitro maturation and development potential of mouse oocytes.关键词
半胱氨酸/小鼠卵母细胞/体外培养/谷胱甘肽/线粒体内膜电位Key words
Cysteine / Mouse oocytes/ In vitro maturation! Glutathione, Mitochondrial membrane potential引用本文复制引用
黄品秀,李蓉,林洪波,刘波澜,赵善飞..抗氧化剂半胱氨酸对小鼠卵母细胞体外成熟及发育潜能的影响[J].生殖医学杂志,2012,21(3):252-256,5.
