天津科技大学学报2012，Vol.27Issue(2)：39-43,78,6.
油脂中总不饱和脂肪酸的紫外光谱测定
Detection of All Unsaturated Fatty Acids in Oil Based on Ultraviolet Spectra
摘要
Abstract
The unsaturated components mainly in 7 kinds of vegetable oil were studied with ultraviolet spectra and analyzed against animal oil. The testing method was thus determined. 0.2% of the original oil with cyclohexane was first of all prepared. Comparisons of ultraviolet curves' features with similarity is an indicator showed that the method had excellent repro-ducibility and differentiae. The common peaks were identified with W testing method, and then all unsaturated fatty acids in different oil were recognized in spectrum and their relative contents were obtained by applying the principles of the conjugate structures of the compounds analyzed with ultraviolet spectra. The analytical results showed that the unsaturated fatty acids were the most abundant in perilla oil and walnut oil, though the content of fatty acid of unsaturated double bond number 4 in perilla oil was 1.5 times as much as in walnut oil, and this kind of fatty acid was the major of all unsaturated fatty acid in olive oil. The content of all unsaturated fatty acids in sunflower seed oil, corn oil, and peanut oil decreased successively, and no unsaturated fatty acids were detected in animal oil. The ultraviolet spectra characteristic of unsaturated fatty acids weakened obviously in the oil samples, after being pre-treated with alkali, saponification,or transesterification. Therefore, this is an easy, fast, accurate method to determine the unsaturated fatty acids in oil.关键词
紫外光谱/油脂/不饱和脂肪酸/紫苏油Key words
ultraviolet spectra/ oil/ unsaturated fatty acids/ perilla oil分类
化学化工引用本文复制引用
赵娟,杨志岩,闫仲丽,降升平..油脂中总不饱和脂肪酸的紫外光谱测定[J].天津科技大学学报,2012,27(2):39-43,78,6.基金项目
与天津出入境检验检疫局合作项目(0000102) （0000102）
