天津科技大学学报2012，Vol.27Issue(3)：1-5,5.
枯草芽孢杆菌谷氨酰胺转氨酶的异源表达
Heterogeneous Expression of Bacillus subtilis Transglutaminase
摘要
Abstract
Gene Sbtg with SD sequence and signal peptide AS0949 Corynebacterium glutamicum was obtained from the genomic DNA of wild type Bacillus subtilis though PCR amplification. It was then inserted into an E. coli-C. glutamicum shuttle vector pXMJ19 to construct expression plasmid pXMJ19-Sbtg. The recombined plasmid was then transformed into Corynebacterium glutamicum ATCC13032. Induced by IPTG.the fermentatiom broth had a cross-linking ability. The result indicated that gene Sbtg was a secretion expression.关键词
谷氨酰胺转氨酶/谷氨酸棒状杆菌/信号肽/分泌表达Key words
transglutaminase/ Corynebacterium glutamicum/ signal peptide/ secretion expression分类
生物科学引用本文复制引用
刘凯,刘逸寒,张艳,田耀,路福平..枯草芽孢杆菌谷氨酰胺转氨酶的异源表达[J].天津科技大学学报,2012,27(3):1-5,5.基金项目
“863"“十二五”农村领域国家科技计划课题资助(2011AA 100905-4) （2011AA 100905-4）
国家自然科学基金资助项目(21076159) （21076159）
