天津中医药2012，Vol.29Issue(3)：266-269,4.
实验性糖尿病鼠大血管病变TGF-β1和CTGF的表达及中药的干预作用
Expression of TGF-β1 and CTGF in rats with experimental diabetic macroangiopathy and intervention role of traditional Chinese medicine
摘要
Abstract
[Objective] To explore the pathogenesis mechanism of macroangiopathy in diabetic patients trough observing the expression of TGF-β1 and CTGF in rats with experimental diabetic macroangiopathy and decide the target in preventing and treating the macroan-giopathy in patients with diabetes through observing the preventing effect of TCM. [Methods] Among eighty male SD rats seventy were induced with diabetes (DM). They were divided into 5 groups: model group (n=14), high, medium and low dose of traditional Chinese medicine groups (n=14), melbine group (n—14). All rats in these six groups were giving with ordinary feed for 12 weeks. After that, arteria cruralis was obtained and the site and change of expression of CTGF and TGF-β1 in which were observed. [Results] TGF-β1 was mainly expressed in the cytoplasm. It is strong positive in model group. In Chinese medicine intervention groups the expression of TGF-β1 was declined obviously (P<0.01). Melbine has influence on the expression of TGF-β1, but the comparison with high dose of traditional Chinese medicine group has significant difference (P<0.05). A large of brown-lyellow material with strong positive reaction (CTGF) could be seen in cytochylema in submucosa of blood vessels of model group. But the CTGF expression was all significantly lowered in cell of blood vessels in drug intervention groups (P<0.01). The difference of expression between high dose group and Western medicine control group was statistically different. [Conclusion] 1 )The onset of diabetic angiopathy may be concerned with fibrosis. 2) Modified Taohe Chengqi Decoction can reduce the expression of Fibrosis factor TGF-β1 and CTGF in rats with experimental diabetic macroangiopathy. It can improve macroangiopathy in DM and has a dose-ldependent relationship.关键词
2型糖尿病/大血管病变/纤维化/中药/结缔组织生长因子/转化生长因子-β1/免疫组化Key words
diabetes mellitus 2 macroangiopathy/ macroangiopathy fibrosis/ traditional Chinese medicine/ CTGF/ TGF-β1/ immunohisto-chemistry分类
医药卫生引用本文复制引用
王军,徐阳,袁向科,张学勇,丁志明..实验性糖尿病鼠大血管病变TGF-β1和CTGF的表达及中药的干预作用[J].天津中医药,2012,29(3):266-269,4.基金项目
天津市应用基础研究计划项目(09JCYBJC12500). （09JCYBJC12500）
