天津中医药2012，Vol.29Issue(3)：274-277,4.
PESV干预K562细胞PI3K/Akt信号通路凋亡调控的研究
Study on PI3K/Akt messenger passage and apoptosis controlling in PESV-preconditioned K562 cells
摘要
Abstract
[Objective] To investigate the effect of PESV on the expression of massage protein PI3K / Akt, apoptosis regulators Bcl-2 and Bad expression in K562 cells. [Methods] In vitro cultured K562 cells preconditioned with PESV for different times were determined for cell apoptosis rate with cytoflowmeter, the level of P13K and p-AKT protein with Western blot and Bcl-2, Bad mRNA level by realtime fluorescence quantitative RT-PCR. [Results] Compared with the control group, the apoptosis rate of PESV treated K562 cells was significantly increaseds, the expression of PI3K and p-Akt and anti-apoptosis-related gene Bcl-2 mRNA was decreased; the expression of pro-apoptotic gene Bad mRNA was increased. [Conclusion] PESV may regulate the expression of Bcl-2 and Bad, inhibit the proliferation of K562 cells and promote their apoptosis by reducing the expression of PI3K and Akt signaling protein.关键词
PESV/K562细胞系/凋亡/PI3K/Akt/Bcl-2/BadKey words
PESV/ K562 cell lines/ apoptosis/ PI3K/ Akt/ Bcl-2/ Bad分类
医药卫生引用本文复制引用
张蕾,杨文华,于文俊,郝征,张佳..PESV干预K562细胞PI3K/Akt信号通路凋亡调控的研究[J].天津中医药,2012,29(3):274-277,4.基金项目
教育部博士点基金项目(20091210110004). （20091210110004）
