天然产物研究与开发2012，Vol.24Issue(5)：581-589,604,10.
牦牛肝蛋白分离纯化及其抗氧化活性研究
Isolation and Purification of Proteins in Liver of Yak (Bos grunniens) and Its Antioxidant Activities
柳青海 1郭洁 2李天才1
作者信息
- 1. 中国科学院西北高原生物研究所,西宁810008
- 2. 中国科学院研究生院,北京100049
- 折叠
摘要
Abstract
Seven proteins ( BGP1,BGP2,BGP3,BGP4,BGP5,GBP6,and BGP7) were isolated respectively from the liver of yak (Bos grunniens) by homogenizing,step precipitating with ammonium sulfate,ion-exchange chromatography on DEAE-52 and gel filtration chromatography on Sepbhadex G-200.Each protein gave a single band on SDS-PAGE and the molecular weight of those proteins were from 97.2 KD to 116 KD.The anti-oxidant activities of proteins were evaluated using the method of chemistry luminescence,including 1,1-dipheny1-2-picrylhydrazyl (DPPH ·),superoxide (O2(..))and hydroxyl (· OH) radical scavenging assays.The proteins could scavenge superoxide radical,hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH ·).Especially,the effects of BGP5 and BGP1 on scavenging superoxide free radical were obvious,with scavenging ability 82.91％ and 72.92％ at the concentrations of 250 mg/L.The results indicated that proteins may be exploited as health food or functional food.关键词
牦牛/肝蛋/纯化/抗氧化活性Key words
yak/liver protein/purification/antioxidation分类
生物科学引用本文复制引用
柳青海,郭洁,李天才..牦牛肝蛋白分离纯化及其抗氧化活性研究[J].天然产物研究与开发,2012,24(5):581-589,604,10.
