天然产物研究与开发2012，Vol.24Issue(5)：631-634,609,5.
白术多糖WAM-1结构的色谱分析和原子力显微镜观察
Chromatographic Analysis and Atomic Force Microscope Observation of Polysaccharide Extracted from Atractylodes macrocephala Koidz.
摘要
Abstract
Polysaccharides extracted from the stem of Atractylodes macrocephala Koidz by hot water,were fractionated by DEAE-52 cellulose chromatography,and purified by Sephadex G-200 gel filtration chromatography to obtain a fraction, named WAM-1. HPLC and GC analysis showed that WAM-1 was a homogeneous and consisted of glucose and galactose in the molar ratio of 3. 01: 1. The molecular morphology of WAM-1 was observed under an atomic force microscope (AFM).The results showed that WAM-1 existed in different forms with different concentrations. The concentration of polysaccharide had effects on the conformation and form of chain interactions, which might be related to the interaction of intramolecular and intermolecular hydrogen bonds. At 10 μg/mL,the morphology of WAM-1 was observed clearly in rigid chains with many branches.关键词
白术多糖/分离纯化/原子力显微镜Key words
polysaccharide/purification/atomic force microscope分类
医药卫生引用本文复制引用
伍乐芹,姜绍芬,张静..白术多糖WAM-1结构的色谱分析和原子力显微镜观察[J].天然产物研究与开发,2012,24(5):631-634,609,5.基金项目
国家自然科学基金项目(10874108) （10874108）
陕西省自然科学基础研究计划(SJ08A16) （SJ08A16）
