生态环境学报2012，Vol.21Issue(4)：775-779,5.
一株聚丁二酸丁二醇酯降解菌的分离鉴定
Isolation and identification of a strain degradating poly (butylene succinate)
摘要
Abstract
The Poly (butylene succinate) (PBS) is a typical agricultural biodegradable materials which have been used for agricultur-al production. The microorganism which could degrade the PBS polymers was separated from Xi'an suburb by the filter paper meth-od, and one strain was successfully obtained which had the most obvious effect, in 30 days it could make the PBS polymer number average molecular weight reduced by 19.80%. Then the strain was identified by general morphological, bio-chemical characteristics and 16S rDNA sequence comparative analysis. The results showed that its physiological and biochemical characteristics was similar to Pseudomonas aeruginosa. And the 16S rDNA sequence of the strain had similarity of 99% with Pseudomonas aeruginosa 39016 contig 00492. In addition to general morphological and bio-chemical characteristics, the strain was identified as one of subspecies of Pseudomonas aeruginosa. The isolation and identification of the strain has established for further biological study of PBS polymer degradation characteristics and mechanism.关键词
铜绿假单胞菌/鉴定/16S rDNA/聚丁二酸丁二醇酯Key words
Pseudomonas aeruginosa/ identification/ 16SrDNA/ PBS分类
资源环境引用本文复制引用
张敏,沈颖辉,李成涛,张欢,张蕊,胡小平..一株聚丁二酸丁二醇酯降解菌的分离鉴定[J].生态环境学报,2012,21(4):775-779,5.基金项目
国家自然科学基金项目(21144008) （21144008）
陕西科技大学科研创新团队资助项目(TD10-01) （TD10-01）
