外科理论与实践2012，Vol.17Issue(3)：248-251,4.DOI:10.3969/j.issn.1007-1096.2012.03.012
Hedgehog信号通路相关蛋白在人胰腺癌SW1990耐药株中的表达及意义
Expression and functional significance of hedgehog signal pathway members in drug-resistant SW1990 pancreatic cancer cell line
姚捷 1苗毅 2安勇 2卫积书 2李强 2吴峻立 2蒋奎荣 2戴存才 2钱祝银 2徐泽宽2
作者信息
- 1. 江苏省苏北人民医院肝胆胰外科,扬州 225001
- 2. 南京医科大学第一附属医院胰腺外科中心,南京 210009
- 折叠
摘要
Abstract
Objective To investigate the difference of protein expression in hedgehog signal pathway in parental and gemcitabine-resistant pancreatic cancer cell line SW1990. Methods Gemcitabine-resistant cell line SW1990 was established by drag concentration step-elevation method. IC50 to gemcitabine in parental and gemcitabine-resistant cells were determined by MIT. mRNA expression levels of Shh, SMO and Gli-1 were determined by real-time PCR. Protein expression level of Shh, SMO and Gli-1 were determined by Western blot. Results IC50 to gemcitabine was (232.2± 12.3) μmol/L in drug-resistant cells and (3.1 ±0.2) (tmol/L in parental cells. mRNA expression of Shh and Gli-1 in gemcitabine-resistant cells increased (12.07±1.71) and (4.15±0.42) fold, respectively, when compared with that in parental cells. mRNA expression of SMO was detectable only in gemcitabine-resistant cells, but not in parental cells. Western blot showed a high expression levels of SMO and Gli-1 proteins in gemcitabine-resistant cells. Conclusions Some members of hedgehog signal pathway are highly expressed in gemcitabine-resistant pancreatic cancer cells. Targeting iherapy against hedgehog pathway may contribute lo overcome gemcitabine-resistant in pancreatic cancer.关键词
胰腺癌/耐药细胞株/Hedgehog信号通路Key words
Pancreatic cancer/ Drug-resistant cell line/Hedgehog signal pathway分类
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姚捷,苗毅,安勇,卫积书,李强,吴峻立,蒋奎荣,戴存才,钱祝银,徐泽宽..Hedgehog信号通路相关蛋白在人胰腺癌SW1990耐药株中的表达及意义[J].外科理论与实践,2012,17(3):248-251,4.
