外科理论与实践2012，Vol.17Issue(3)：252-256,5.DOI:10.3969/j.issn.1007-1096.2012.03.013
胆囊结石病人成纤维细胞生长因子-19的血浆含量及胆囊黏膜表达特点
Fibroblast growth factor-19 in gallstone patients: plasma content and expression of gallbladder mucosa characteristics
摘要
Abstract
Objective To evaluate the relation between fibroblast growth factor (FGF)-19 and gallbladder raotility function by measuring the serum level of FGF-19 and the expression of FGF-19 mRNA in gallbladder mucosa from patients with gallstone disease. Methods Nine male and 18 female patients with gallstone disease were recruited. FGF-19 mRNA expressions in gallbladder mucosa were determined by real-time PCR. Plasma FGF-19 level at fasting, 1.5 h and 3.0 h after fat diet were assayed by ELISA, the gallbladder volume at the corresponding lime points were measured by B-type ultrasonic scanning. Results According to gallbladder ejection fraction (EF) at 1.5 h after fat diet, the patients were divided into 3 groups. For normal contraction group (EF > 60%, n= 5), low contraction group fEF (30 ~ 60)%, n=15] and non contraction group (EF < 30%, n=7), EF at 3.0 h after fat-diet were (67.2±3.4)%, (48.2±2.4)%, and (17.0±3.5)%, respectively, P < 0.01. Gallbladder refilling rate in normal contraction group (82±12)% was higher than low contraction and non contraction group [(37±11)% and (7±7)%], P = 0.006. FGF-19 mRNA expression in gallbladder mucosa was significantly lower in the non contraction group (130±7I) than in the normal contraction group and the low contraction group (222±82 and 254±50), P<0.05. Fasting plasma FGF-19 level in non contraction group [(62.1±20.0) pg/ml.] was lower than the other groups[normal contraction group:(157.9±22.3) pg/mL], low contraction group: [(175.8±25.6) pg/mL], P< 0.05. Conclusions Gallbladder contraction and refilSing function were impaired in subgroups of the gallstone patients. Changes of plasma FGF-19 level may reflect the abnormalities of gallbladder contraction and refilling.关键词
成纤维细胞生长因子-19/胆囊收缩舒张/胆囊结石病/胆囊容积Key words
Fibroblast growth factor-19/ Gallbladder contraction and refilling/ Cholelithiasis/ Gallbladder volume分类
医药卫生引用本文复制引用
雷铭,蒋兆彦,曹奕鸥,陈红燕,蔡劬,计骏,王明亮,韩天权,张圣道..胆囊结石病人成纤维细胞生长因子-19的血浆含量及胆囊黏膜表达特点[J].外科理论与实践,2012,17(3):252-256,5.基金项目
国家自然科学基金(81070367,30700310) （81070367,30700310）
